Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 500g
PIPES | Purity: 99% | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 500g
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Research Products International Corp TRIS ACETATE ULTRA PURE, 100 GRAM
Moisture: <0.3%
pH (0.1M, H2O) @25°C: 6.0-7.0%
Heavy Metals: 0.001%
Chem-Impex International, Inc. MOPS sodium salt | 71119-22-7 | MFCD00064350 | 1KG
MOPS sodium salt, 71119-22-7, MFCD00064350, 1KG
![Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre-Filled Tubes, Sterile, 100 Tubes per Case](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10420-100.0.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre-Filled Tubes, Sterile, 100 Tubes per Case
PBS (Phosphate-Buffered Saline) is a balanced salt solution that helps maintain the pH and osmolarity of experimental systems, making it ideal for various biological and biochemical experiments.
1X Solution refers to a diluted concentration of a particular solution. In the case of PBS, a 1X solution means that it is prepared by diluting the concentrated stock solution to a specific ratio, usually 1 part stock solution to 9 parts water or another suitable solvent. 1.0ml Pre-Filled Tubes are small tubes that come pre-filled with a specified volume of liquid, in this case, 1.0 milliliters of PBS. These tubes are convenient and sterile, ensuring the integrity and purity of the solution. They are commonly used in laboratory settings to provide a controlled and standardized amount of PBS for various applications.
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100 g
PIPES | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 100 g
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 1 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Medchemexpress LLC 2-Methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.9% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptors agonist. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptors agonist
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation
![Research Products International Corp TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60015-1000.03.JPG-250.jpg)
Research Products International Corp TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter
TAE 50X Buffer is a concentrated solution of Tris, acetic acid, and EDTA used in nucleic acid electrophoresis. When diluted, it maintains pH (~8.3) and low ionic strength, supporting clear DNA/RNA separation. EDTA chelates metal ions, helping prevent nucleic acid degradation during electrophoretic analysis in molecular biology workflows.
Substance Name: TRIS Acetate EDTA 50X Solution
Solution contains:
Acetic Acid: 1 M
EDTA, Disodium Salt Dihydrate: 50 mM
Hampton Research HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml
HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Research Products International Corp PIPES FREE ACID ULTRAPURE, 1 KILOGRAM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
